Part:BBa_K3755015

mRuby2 red fluorescent protein

monomeric red fluorescent protein derived from mRuby, with improved photophysical properties

Introduction

Ever since fluorescent proteins were introduced into biology research, it's widely used in cell imaging and biosensors. mRuby2 is a red fluorescent protein. It has a better brightness and fluorescence resonance energy transfer (FRET) dynamic range. And mRuby2 functions well in a variety of fusions. In our project, mRuby2 is linked to carboxyl-terminal of Piezo1 and Piezo1.1, to obtain whether the plasmids containing Piezo1/Piezo1.1 are transfected into our HEK293 cells successfully.

Cell imaging

We cotransfected Piezo1.1 and GCaMP6m into HEK293 cells. Cell imaging results are shown below. A shear force was applied to the cells by sucking out the culture medium.

Figure2:a.Red fluorescence of Piezo1.1-mRuby2, indicating the transfection of Piezo1.1    b.Green fluorescence of GCaMP after the activation of Piezo1.1 by force, 20X objective, indicating the transfection of pEGFP-GCaMP    c.Merge of the green channel and red channel, indicating the cotransfection of GCaMP and Piezo1.1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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